Figure 5. Acetate and ROS contribute towards cell death.

(A) Aerobically grown cultures of S. aureus UAMS-1 (TSB-35 mM glucose or TSB-35 mM glucose 50 mM MOPS, pH 7.3) were shifted to an anaerobic chamber after 24 h of growth. Cell viability was determined daily by counting colonies (cfu/ml) on solid media incubated anaerobically at 37°C. Culture aliquots were sampled 48 h post-shift to anaerobiosis and double stained with HPF/CTC (B) and subjected to a TUNEL assay (C).