Table 1.
Chemical and Physical Properties of Nerve-Targeting Fluorophores.
| Fluorophore | MW (Da) |
LogD, pH 7.4 |
PSA (Å2) |
HBD/ HBA |
Rotat. Bonds | λ (M-1cm-1) |
Abs (nm) |
Em (nm) |
SBR | |
|---|---|---|---|---|---|---|---|---|---|---|
| Ex vivo | In vivo | |||||||||
| BMB | 364.06 | 4.84 | 61.27 | 2/3 | 5 | 52,800 | 397 | 498 | + | + |
| Oxazine 1 | 423.89 | 0.33 | 27.84 | 0/3 | 5 | 196,000 | 655 | 670 | - | - |
| Oxazine 4 | 395.84 | 3.38 | 47.59 | 2/3 | 3 | 143,000 | 616 | 635 | ++ | +++ |
| Oxazine 170 | 431.87 | 3.94 | 47.59 | 2/3 | 3 | 69,000 | 625 | 650 | + | + |
| Oxazine 750 | 469.92 | 4.67 | 38.80 | 1/3 | 1 | 44,000 | 676 | 684 | + | - |
| Rhodamine 800 | 495.95 | 0.43 | 39.27 | 0/3 | 0 | 68,000 | 697 | 710 | + | - |
MW = molecular weight; PSA = polar surface area; HBD/HBA = H bond donors/acceptors; Rotat. Bonds = rotational bonds; λ = extinction coefficient; Abs = peak absorbance; Em = peak emission. The SBR of each nerve relative to the neighboring adipose tissue was quantified and labeled as -, < 1; +, 1 to 2; ++, 2 to 3; and +++, > 3.