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(a) LC3II/I ratio was higher in elder (>60 years old) as compared to younger (<60 years old) subjects. (b) Conversely, p62 was lower in older subjects. These findings reflect the occurrence of an age-dependent blockage in autophagy as occurring in the TM. (c) Evaluation of LC3I, LC3II, and p62 proteins in the TM by Western blot. Protein amounts were compared at different age categories.
