Fig. 4.

CCN5 colocalizes with cortactin in podosomes of several classic morphologies, and CCN2 does not a-c Podosomes induced by stimulation of VSMCs with 2 μM PDBU phorbol ester phorbol 12,13-dibutyrate (PDBu) for 1 h. Cells were then fixed and stained with antibodies for cortactin (green), CCN5 (red), and DAPI (blue). Bars = 10 μm d Podosomes induced by stimulation of VSMCs with 0-2 μM PDBU phorbol ester phorbol 12,13-dibutyrate (PDBU) for 1 h. Percent of cells containing podosomes positive for CCN5 and/or cortactin were counted and graphed against increasing PDBU concentration. Error Bars = standard deviation, n = 3. e Podosomes induced by stimulation of VSMCs with 2 μM PDBU phorbol ester phorbol 12,13-dibutyrate (PDBu) for 1 h. Cells were then fixed and stained with antibodies for cortactin (red), CCN5 (green), and DAPI (blue). Bars = 10 μm