Fig. 7.

Characterization of PSC exosomes. Exosomes isolated from the medium of cultured PSC were analyzed by a Western blot for CD9 and b TEM. qRT-PCR was performed to detect miR-21 or CCN2 mRNA in c exosomes from activated PSC or d exosomes from PSC transfected for 24 h with either CCN2-GFP or miR-21-GFP. Values above the bars in d represent the approximate fold increase in transcript level versus their respective controls which are normalized to a value of 1 and had comparable ΔCT values to those shown in c