FIG. 2.

Overexpression of β-defensin 124 (DEFB124) in RWPE-1 cells. (A) DEFB124 mRNA overexpression. The RWPE-1 cells were transfected with DEFB124-DDK-Myc vector or empty vector, and DEFB124 mRNA expression was determined by reverse transcription-polymerase chain reaction (left) and quantitative real-time polymerase chain reaction (right). ACTB was used as an internal control. Asterisk represents statistical significance at p<0.0001. (B) Upregulation of DEFB124 protein. DEFB124 protein was evaluated by western blot by using antibodies against DEFB124 and Myc. ACTB was used as an internal control.