FIG. 4.

Beta-Defensin 124 (DEFB124)-mediated upregulation of cytokines and chemokines promote chemotactic response of THP-1 monocytes. DEFB124- or DEFB124-mediated cytokines and chemokines induce chemotaxis for THP-1 monocytes. THP-1 cells (1×10⁶ cells) were added to the upper chamber, and the lower chamber contained supernatants from either the empty vector- or DEFB124-DDK-Myc-transfected RWPE-1 cells. The results are presented as a migration index denoting the fold increase of cell migration over the empty vector. Monocyte chemoattractant protein-1 (MCP-1) (100 ng/mL) was used as a positive control. Results are representative of three independent experiments. Asterisk represents statistical significance at p<0.02.