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Sequences of primers for occludin, zona occludens-1, and β-actin
| Tested gene product | Primer sequences | Fragment | |
| Occludin | Upstream | 5’-CCTGTTTAGTTAGGTGAAG-3’ | 156 bp |
| Downstream | 5’-TTCCTGAGAAGGGTTATG-3’ | ||
| ZO-1 | Upstream | 5’-GGGGGATTTATAACTTGGG-3’ | 321 bp |
| Downstream | 5’-CTGGTTGGATGTCTGTGG-3’ | ||
| β-Actin | Upstream | 5’-GCGTGACATTAAAGAGAAGCTG-3’ | 500 bp |
| Downstream | 5’-AGAAGCATTTGCGGTGCAC-3’ | ||
ZO-1: Zona occludens-1.
