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Schematic representation of PCR-ELISA and LAMP-ELISA assays. The biotin-labeled specific oligonucleotide probes are applied onto the surface of streptavidin-coated well and used to hybridize with biotin-labeled LAMP amplicons or biotin-labeled PCR products. After the nonspecific binding LAMP amplicons or biotin-labeled PCR products were removed, the conjugates of streptavidin- horseradish peroxidase (HRP) were added to perform the ELISA detection.
