Fig. 3.

Optimization of loop-mediated isothermal amplification (LAMP) assay for the detection of VP2 sequence from CPV genomic DNA. (A) For the test of optimal temperature, reaction temperature of 65, 63, 61 and 59°C was tested using the genomic DNA extracted from 100 µl of CPV suspension (10⁶ TCID₅₀/ml) as template. (B) For the interactive test of reaction time and template DNA concentration, the CPV genomic DNAs were extracted from 10⁵, 10³ and 10¹ TCID₅₀/ml, and then, each CPV genomic DNA was used in the LAMP reaction for 15, 30, 45 and 60 min. “N” indicated the negative control. “M” indicated the 100-bp ladder DNA marker, and the molecular of partial DNA ladders were also noted.