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. 2013 Dec 12;76(4):509–516. doi: 10.1292/jvms.13-0448

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©2014 The Japanese Society of Veterinary Science

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Fig. 4. — Sensitivity of PCR (A), loop-mediated isothermal amplification (LAMP) (B), LAMP combined with enzyme-linked immunosorbent assay (ELISA) (C) and LAMP combined with lateral flow dipstick (LFD) (D) assays for the detection of VP2 sequence from CPV genomic DNA. The templates of CPV genomic DNA were extracted from CPV suspension ranging from 10⁶ to 10⁻² TCID₅₀/ml. “N” indicated the negative control, and “M” indicated the 100-bp DNA ladder marker.