Table 1. The information of primers and probes used in this study for the sequence detection of VP2 gene of canine parvovirus.
| Primers and probes | Nucleotide position | Sequences (5’→3’) |
|---|---|---|
| Primer sets for LAMP | ||
| CPV-F3 | 1409–1433 | GGGATAAAGAATTTGATACTGACTT |
| CPV-B3 | 1627–1605 | GAGAGGCTCTTAGTTTAGCTTTA |
| Biotin-CPV-FIP (F1C-F2) | 1512–1488/1440–1461 | Biotin-AGGCGCAACTTTTACAAATAATTGAAAGACTTCATGTAAATGCACCA |
| CPV-BIP (B1C-B2) | 1530–1554/1595–1573 | TGATCCTGATGCATCTGCTAATATGTACCTTTCCACCAAAAATCTGA |
| Primer pair for PCR | ||
| Biotin-CPV-F | 1375–1398 | Biotin-AATGTACCACCAGTTTATCCAAAT |
| CPV-R | 1666–1693 | TACTTGGTACATAGTTAAATTGGTTATC |
| Probe for LAMP-ELISA | ||
| Biotin-CPV-probe | 1520–1546 | Biotin-CAAATGAATATGATCCTGATGCATCTG |
| Probe for LAMP-LFD | ||
| FITC-CPV-probe | 1520–1546 | FITC-CAAATGAATATGATCCTGATGCATCTG |