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Polymerase chain reaction amplification products of c-KIT exon 11 from Case 1. Lane M: 50-bp DNA ladder. Lane 1 shows a single band amplified from a normal dog genome, representing the wild-type c-KIT. Lane 2 shows aberrant bands amplified from Case 1. Although the difference of these bands looks like dozens of bases, a 9-bp deletion was subsequently identified in sequence analysis. The upper band may be a heteroduplex of the wild-type and mutant c-KIT.
