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Initial assessment of envelope peripheral protein topology after “tag” or “shave” positive reactions.
| Biotinylation (tagged) | Thermolysin sensitivity (shaved) | Protein at the surface of the organelle |
|---|---|---|
| − | − | ? |
| + | − | yes |
| − | + | yes |
| + | + | yes |
(?) In the absence of biotinylation or sensitivity to thermolysin, although the polypeptide is probably not exposed at the surface of the plastid envelope, one cannot exclude a localization at the OEM.
