Fig. 3.
Adhesion of monocytes from whole blood on surfaces functionalized with anti-CD14 MY4 clone in response to shear stress and antibody concentration. Differentiated capture of monocytes in response to varying shear rates was observed. The maximum cell capture density was also a function of the antibody concentration. Considering reagent cost, we chose to use an antibody concentration of 35 μg/mL and shear stress of 0.3 dyn/cm2 for monocyte depletion. Each data point was repeated in 3 devices spanning different shear stress ranges; the error bars represent standard deviations from these measurements.