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. 2014 Jun 4;21(1):55. doi: 10.1186/1423-0127-21-55

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Copyright © 2014 Arya et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ASC differentiation to adipose, osteocyte and endothelial like cells. Oil Red O staining was used to detect lipid droplet formation during adipogenic differentiation (panel A). The left side image depicts control ASCs grown in M-199 medium while the right one shows ASCs grown in adipogenic differentiation medium. Alizarin Red staining was used to measure calcium deposition during osteogenic differentiation (panel B). The left side image depicts control ASCs grown in base medium M-199 while the right one shows ASCs grown in osteogenic differentiation medium. Endothelial differentiation was measured by CD31 flow cytometry (panel C). Left side is undifferentiated ASCs grown in M-199 medium, the middle is differentiated ASCs grown in EGM2 medium and the right is HUVECs endothelial cell control.