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. 2014 Jun 4;21(1):55. doi: 10.1186/1423-0127-21-55

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Copyright © 2014 Arya et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The effect of bradykinin (Bra), S1P and PGE on the expression of endothelial differentiation markers CD31, vWF and eNOS in ASCs differentiated to endothelial-like cells. Real-Time PCR was performed to evaluate target gene expression. GAPDH was used to normalize target gene expression levels. A no treatment group was used as control to calculate relative expression between treatments groups. Left panel is for CD31, middle panel is for vWF and right panel is for eNOS. A total n = 6 individual ASC cell lines were tested.