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. 2014 Jun 4;21(1):55. doi: 10.1186/1423-0127-21-55

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Copyright © 2014 Arya et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The up-regulation of PI3K in S1P treated ASC-differentiated endothelial cells. Panel A: Western-blot for PI3K and β-actin in ASCs (M-199), ASC-differentiated endothelial cells (EGM2) and S1P treated ASC-differentiated endothelial cells (EGM2 + S1P). Panel B: Analyzed Western-blot data from four ASC cell lines, *indicated statistical significance with p < .05. C: Immunostaining for PI3K and β-actin in ASC-differentiated endothelial cells. Red fluorescence is for PI3K and green fluorescence is for β-actin. Both Western-blot and immunostaining demonstrate the up-regulation of PI3K by S1P in ASC-differentiated endothelial cells.