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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Biochem Soc Trans. 2013 Oct;41(5):1219–1226. doi: 10.1042/BST20130101

Figure 3. Single-molecule FRET of ε conformational changes in F1.

Figure 3

A, B: Photon bursts of FRET-labeled F1, with donor Atto488 attached to γ108 (green traces in lower panels,) and acceptor Atto647N attached to ε99 (red traces in lower panels, labeling efficiency 30%). Grey traces are Atto647N intensities upon direct excitation with 635 nm. C–E: FRET efficiency histograms for FRET-labeled F1, in the presence of 1 mM Mg2+ADP plus 3 mM Pi (C), 1 mM Mg2+AMPPNP (D), or 1 mM Mg2+ATP (E), respectively. See Fig. 1 for label positions. Reference lines are shown at EFRET 0.25, 0.5 and 0.75.