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Box-and-whiskers plot showing the size (A) and Z-potential (B) of amino-modified beads untreated (sample 1), incubated with pure HisCPC and tyrosinase (sample 2), and incubated only with HisCPC (sample 3). In panel B, the inset shows the beads as imaged by fluorescence microscopy. Z-potential measurement (C) and SDS PAGE analysis (inset, staining with zinc) of the protein solution at the end of the immobilization process (samples numbered as in A and B).
