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. 2014 Jun 20;4:5370. doi: 10.1038/srep05370

Figure 5. Schematic representation of the possible tyrosinase-catalyzed immobilization of HisCPC to amino-modified polystyrene beads according to our study.

Figure 5

Crosslinking of HisCPC to the bead can involve Tyr10 1 or Tyr19 that is located within the TEV cleavage site 2, or both 3. A second layer of HisCPC might be immobilized by introducing crosslinks between the nucleophilic residues from the HisCPC core and the N-terminal peptide of a second HisCPC molecule 4, or free tyrosine residues from the N-terminal peptide of a bound HisCPC molecule and either nucleophilic groups from the HisCPC core 5, or tyrosine residues of the N-terminal peptide 6.