Table 1.
Effect of manipulating, experimentally and computationally, subthreshold conductances on resting membrane potential of TC neurons
| Experiment |
Model |
||||||
|---|---|---|---|---|---|---|---|
| RMP |
RMP |
||||||
| Current | Treatment | Before | Aftera | Reference | Treatment | Before | After |
| IKleak | 1 mM MCh (300 nM TTX) | −68.6 ± 2.5 | −55.0 ± 2.5b | Present study | Turn off IKleak | −69.7 | −59.3 |
| 250 μM MCh | 12 ± 6 mV depolarization | Varela and Sherman 2007 | Turn off IKleakc | −71.5 | −62.3 | ||
| INaleak | Turn off INaleak | −69.7 | −77.6 | ||||
| Ih | 10 μM ZD-7288 | −71.1 ± 2.4 | −78.3 ± 1.7d | Present study | Turn off Ih | −69.7 | −77.9 |
| 100 μM ZD-7288 | −71.0 ± 1.0 | −79.0 ± 2.0 | Meuth et al. 2006 | ||||
| HCN2 KO | −69.0 ± 1.0 | −81.0 ± 1.0 | Meuth et al. 2006 | ||||
| −68.0 ± 1.0 | −80.0 ± 1.0 | Ludwig et al. 2003 | |||||
| INaP | 300 nM TTX | −71.0 ± 1.5 | −71.9 ± 1.6e | Present study | Turn off INaPf | −69.7 | −71.5 |
| IKir | 50 μM Ba2+ | −69.0 ± 1.1 | −66.5 ± 0.9g | Present study | |||
| Kir2.2 KO | −69.3 ± 0.7 | −66.8 ± 0.8h | Present study | Turn off IKir | −69.7 | −68.6 | |
| IT | 1-3 μM TTA-P2 | 3.1 ± 0.5 mV hyperpolarization | Dreyfus et al. 2010 | Turn off IT | −69.7 | −72.3 | |
| IA | Turn off IA | −69.7 | −57.2 | ||||
Values are resting membrane potential (RMP; ±SE) in experimental or model-simulated thalamocortical (TC) neurons.
Comparison between wild type and knockout (KO) genotypes in the case of genetic elimination of HCN2 or Kir2.2.
Comparison of RMP before and after application of 1 mM β-methylcholine (MCh) in the presence of 300 nM TTX (n = 7; paired t-test, P = 0.00003).
Comparison of RMP before and after IKleak is turned off, with the sodium conductances previously switched off.
Comparison of RMP before and after application of 10 μM ZD-7288 (n = 10; paired t-test, P = 0.0004).
Comparison of RMP before and after application of 300 nM TTX (n = 18; paired t-test, P = 0.003).
Concomitantly turning off INaP and INa produces similar results.
Comparison of RMP before and after application of 50 μM Ba2+ (n = 17; paired t-test, P = 0.001).
Comparison between wild type (n = 66) and Kir2.2 KO (n = 19; 2-sample t-test, P = 0.02). TTA-P2, 3,5-dichloro-N-[1-(2,2-dimethyl-tetrahydropyran-4-ylmethyl)-4-fluoro-piperidin-4-ylmethyl]-benzamide. See text for current definitions.