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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: J Cell Sci. 2008 Sep 2;121(0 19):3133–3139. doi: 10.1242/jcs.034496

Fig. 3. BAPTA-am loading reversed the disassembly of STIM1 clusters by histamine.

Fig. 3

(A) Representative images of the sensitivity of pre-existing subplasmalemmal STIM1 clusters to histamine and their recovery upon reduction of [Ca2+]cyto (n=6). (B) BAPTA reversed the decomposition of preexisting subplasmalemmal STIM1 clusters upon histamine stimulation independently from extracellular Ca2+ (n=6). (C,D) Cytosolic Ca2+ signaling (C) and kinetics of subplasmalemmal STIM1 clustering (D) in response to 100 μM histamine in the presence of 2 mM extracellular Ca2+ in YFP-STIM1-expressing cells that were loaded with either fura-2-am (n=6) or with fura-2-am and BAPTA-am (n=6). *P<0.05 versus the respective compared data set.