Fig. 8.

Clotrimazole and CITCO produce CAR3-dependent activation of the DR-4 × 3 reporter in HEK293 cells. Transfections were performed as described in Fig. 1, A and B, with plasmids indicated in the figure. On day 1, HEK293 cells were transfected. Eighteen hours after transfection, cells were treated with indicated chemicals (CLOT, clotrimazole). Twenty-four hours after chemical treatment, cells were harvested and luciferase activity was measured as described under Materials and Methods. CMV2 and 3.1 represent empty expression vectors. Data are presented as normalized and adjusted values in which the activity of the DMSO-treated CMV2/RXR data point was set to 1. Each data point represents the mean ± S.E. of four separate transfections.