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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: Mol Pharmacol. 2005 Aug 11;68(5):1239–1253. doi: 10.1124/mol.105.013417

TABLE 1.

pTracer-CMV2 (Invitrogen, Carlsbad, CA) clones

The parent plasmid contains a CMV promoter that yields robust expression of cloned sequences when transfected into mammalian cells. This plasmid is referred to in the text as “CMV2”.

Name PCR Primers Amino
Acids		 Restriction
Sites
CAR1 FP:GATCGAATTCGTCATGGCCAGTAGGGAAGATGAG 1–348 EcoR1/EcoRV
RP:GATCGATATCTCAGCTGCAGATCTCCTGGAGCCAG
CAR3 FP:GATCGAATTCGTCATGGCCAGTAGGGAAGATGAG 1–353 EcoR1/EcoRV
RP:GATCGATATCTCAGCTGCAGATCTCCTGGAGCCAG

FP, forward primer; RP, reverse primer.