Figure 3.

Effects of hexane fraction from the root extract on the intracellular lipid accumulation and cell viability. (A) Results are a mean value ± S.D. of six-replicate measurements. Bar graphs represent the relative intracellular lipid contents. Asterisks indicate a significant difference at p <0.05 from the untreated control (0 μg/mL). Percentages of cell viability are shown as a line graph. Crosses indicate a significant difference at p <0.05 from the untreated control (0 μg/mL). The standard TNFα at 10 ng/mL gave 9.17 ± 3.98% of relative lipid content in these experiments. (B) The relative amounts of accumulated lipid are shown as a mean value ± S.D. of five-replicate measurements for each period that the fraction (1,000 μg/mL) was present in the culture medium. Asterisks indicate a significant difference at p <0.05 from the untreated control (0 μg/mL) at Day 8.