Table 1.

Primer sequences for Real-Time PCR

Gene	Forward sequence	Accession No.
(product)	Reverse sequence
Actin	5′-CAGAAGGAGATTACTGCTCTGGCT-3′	NM_007393
(93 bp)	5′-GGAGCCACCGATCCACACA-3′
ACC	5′-GGACCACTGCATGGAATGTTAA-3′	AY451393
(73 bp)	5′-TGAGTGACTGCCGAAACATCTC-3′
Adiponectin	5′-GTTCCCAATGTACCCATTCGC-3′	NM_009605
(88 bp)	5′-TGTTGCAGTAGAACTTGCCAG-3′
Adipor1	5′-TCTTCGGGATGTTCTTCCTGG-3′	NM_028320
(104 bp)	5′-TTTGGAAAAAGTCCGAGAGACC-3′
C/EBPα	5′-TGGACAAGAACAGCAACGAGTAC-3′	AM_007678
(257 bp)	5′-GCAGTTGCCCATGGCCTTGAC-3′
FABP4	5′-AGCATCATAACCCTAGATGG-3′	NM_024406.2
(115 bp)	5′-CATAACACATTCCACCACCAGC-3′
FAS	5′- TGCTCCCAGCTGCAGGC -3′	AF_127033
(91 bp)	5′-GCCCGGTAGCTCTGGGTGTA-3′
GLUT4	5′-CTGCAAAGCGTAGGTACCAA-3′	BC014282
(87 bp)	5′-CCTCCCGCCCTTAGTTG-3′
IRS1	5′-CCAGAGTCAAGCCTCACACA-3′	NM_010570.4
(179 bp)	5′-GAAGACTGCTGCTGCTGTTG-3′
LPL	5′-AGGGCTCTGCCTGAGTTGTA-3′	NM_008509
(199 bp)	5′-AGAAATCTCGAAGGCCTGGT-3′
MEST	5′-GTTTTTCACCTACAAAGGCCTACG-3′	NM_008590
(52 bp)	5′-CACACCGACAGAATCTTGGTAGAA-3′
PDK4	5′-GAGAAGAGCCCAGAAGACCA-3′	NM_013743
(134 bp)	5′-TCCACTGTGCAGGTGTCTTT-3′
PPARγ	5′-AGGCCGAGAAGGAGAAGCTGTTG-3′	NM_011146
(276 BP)	5′-TGGCCACCTCTTTGCTGTGCTC-3′
PPARγ1	5′-AAGATTTGAAAGAAGCGGTGAAC-3′	NM_001127330
(116 bp)	5′-CAATGGCCATGAGGGAGTTAG-3′
SIRT1	5′-GACGACGAGGGCGAGGAG-3′	NM_019812
(79 bp)	5′-ACAGGAGGTTGTCTCGGTAGC-3′

ACC, acetyl-CoA carboxylase; Actin, cytoplasmic β-actin; Adipor1, adiponectin receptor-1; C/EBPα, CCAAT/enhancer binding protein transcription factor-α; FABP4, fatty acid binding protein-4; FAS, fatty acid synthase; GLUT4, glucose transporter-4; IRS1, insulin receptor substrate-1; LPL, lipoprotein lipase; MEST, mesoderm specific transcript; PDK4, pyruvate dehydrogenase kinase-4; PPARγ, peroxisome proliferator activated receptor-γ; PPARγ1, peroxisome proliferator activated receptor-γ1; SIRT1, sirtuin (silent mating type information regulation 2 homolog)-1; bp, base pairs.