Figure 2.

Leakage data for CHAPS (open squares), C₁₂EO₈ (open circles), and mixtures thereof (solid symbols) acting on 300 μM POPC vesicles. (Bars in symbols distinguish independent data sets.) (A) Dye efflux after 1 h, E, as a function of the concentration of CHAPS (for pure CHAPS and mixtures) or C₁₂EO₈. (Solid symbols) Mixtures of three series with constant C₁₂EO₈ of 163 μM (left-pointing triangles), 133 μM (diamonds), and 80 μM (right-pointing triangles), respectively, and variable CHAPS (see abscissa). (Solid lines) Values calculated using Eq. 3 assuming additive action (CI = 1) (dotted lines are to guide the eye only). The fact that the points for mixtures are at lower concentration ( see corresponding solid curves) implies synergistic action. (B) E as a function of the fluorescence lifetime of entrapped dye, τ_E, on a reciprocal scale. To see this figure in color, go online.