Leakage data for surfactins (SFs, open squares), C12EO8 (open circles), and mixtures thereof (solid symbols) acting on 30 μM POPC vesicles. (Bars in symbols distinguish independent data sets.) (A) Dye efflux after 1 h, E, as a function of the concentration of SF (for pure SF and mixtures) or C12EO8. (Solid symbols) Mixtures of four series with constant C12EO8 of 10 μM (pentagons), 25 μM (right-pointing triangles), 35 μM (diamonds), and 45 μM (left-pointing triangles), respectively, and variable SF (see abscissa). (Solid lines) Values calculated using Eq. 3 assuming additive action (CI = 1) (dashed lines are to guide the eye only). Concerted action is additive, within error. (B) E as a function of the fluorescence lifetime of entrapped dye, τE, on a reciprocal scale. To see this figure in color, go online.