Figure 4.

Leakage data for fengycins (FEs, open squares), C₁₂EO₈ (open circles), and mixtures thereof (solid symbols) acting on 30 μM POPC vesicles. (A) Dye efflux after 1 h, E, as a function of the concentration of FE (for pure FE and mixtures) or C₁₂EO₈. (Solid symbols) Mixtures of three series with constant C₁₂EO₈ of 10 μM (right-pointing triangles), 20 μM (diamonds), and 30 μM (left-pointing triangles), respectively, and variable FE (see abscissa). (Solid lines) Values calculated using Eq. 3 assuming additive action (CI = 1) (dotted lines are to guide the eye only). Concerted action is strongly synergistic due to the disappearance of the plateau region seen for FE alone. (B) E as a function of the fluorescence lifetime of entrapped dye, τ_E, on a reciprocal scale. To see this figure in color, go online.