Fig. 1.

Association of ApoAI and eNOS. HUVEC were incubated in serum-free medium containing ApoAI (50 μg/ml) for 1 h. After crosslinking, cells were lysed and harvested before immunoprecipitation with anti-ApoAI antibodies probed by Western blotting for eNOS (A), anti-eNOS antibodies probed by Western blotting for ApoAI (B), and anti-ApoAI antibodies probed by Western blotting for caveolin-1 (cav-1) (C). +, Positive control for immunoblot protein; 1, crosslinked immunoprecipitated complexes. eNOS- and mock-transfected CHO cell lysates were Western blotted for eNOS expression (D), and ligand blotting with ApoAI of the same experiment shows immunoblot for ApoAI (E), where lanes are control (C), mock (-), and eNOS (+). Results are indicative of three separate experiments.