Fig. 6.

Transgenic Tbx10 overexpression causes CL/P. (A and B) Wholemount in situ hybridization analysis showed ubiquitous overexpression of Tbx10 transcripts in CMVβ-Tbx10 transgenic embryos at E10.5 (B) compared with the highly restricted hindbrain expression in wild-type littermates (A). (C and D) Frontal views of a wild-type E18.5 embryo (C) and of a littermate transheterozygous for Dc and the CMVβ-Tbx10 transgene (D). The transheterozygous embryo exhibited bilateral cleft lip and open eyelids. (E and F) Palatal views of wild-type (E) and transheterozygous mutant (F) upper jaws showed cleft primary and secondary palates in the mutant.