Table 4.
Influence of Serum on Biological Process Ontologies in Primary and Immortalized Human Meibomian Gland Epithelial Cells
|
Ontology |
Undifferentiated vs. Serum |
SFM vs. Serum |
||||||
|
Pri Genes |
Pri
z-Score |
Imm Genes |
Imm
z-Score |
Pri Genes |
Pri
z-Score |
Imm Genes |
Imm
z-Score |
|
| Upregulation | ||||||||
| Anatomical structure development | 349 | 2.64 | 334 | 3.35 | 256 | 4.28 | 291 | 4.4 |
| Apoptosis | 195 | 6 | 184 | 6.13 | 132 | 5.21 | 159 | 6.43 |
| Apoptotic mitochondrial changes | 11 | 3.68 | 9 | 2.89 | 10 | 4.69 | 8 | 2.97 |
| Cell adhesion | 117 | 4.49 | 96 | 2.78 | 97 | 6.52 | 103 | 5.88 |
| Cell death | 209 | 5.85 | 199 | 6.17 | 142 | 5.16 | 166 | 5.88 |
| Cell differentiation | 237 | 2.25 | 223 | 2.49 | 162 | 2.38 | 195 | 3.38 |
| Cell migration | 87 | 4.13 | 76 | 3.31 | 62 | 4.11 | 71 | 4.4 |
| Cell morphogenesis | 86 | 2.81 | 75 | 2.07 | 62 | 3.11 | 73 | 3.64 |
| Cellular response to stimulus | 500 | 4.25 | 446 | 3.07 | 337 | 3.99 | 387 | 4.31 |
| Epidermis development | 42 | 4.72 | 46 | 6.23 | 28 | 3.86 | 27 | 2.82 |
| Epithelium development | 65 | 4.3 | 61 | 4.28 | 46 | 4.09 | 49 | 3.67 |
| Induction of apoptosis | 60 | 3.99 | 59 | 4.47 | 36 | 2.44 | 54 | 5.16 |
| Induction of programmed cell death | 60 | 3.95 | 59 | 4.43 | 37 | 2.62 | 54 | 5.11 |
| Organ morphogenesis | 76 | 2.24 | 75 | 2.84 | 53 | 2.29 | 72 | 4.24 |
| Programmed cell death | 196 | 5.99 | 185 | 6.12 | 134 | 5.36 | 160 | 6.45 |
| Proteolysis | 132 | 5.22 | 130 | 5.98 | 87 | 4.18 | 108 | 5.59 |
| Signal transduction | 430 | 4.31 | 391 | 3.71 | 294 | 4.3 | 328 | 3.95 |
| Signaling | 453 | 3.21 | 412 | 2.65 | 314 | 3.77 | 347 | 3.11 |
| Tissue development | 136 | 5.27 | 128 | 5.33 | 98 | 5.39 | 111 | 5.61 |
| Vesicle-mediated transport | 115 | 4.36 | 103 | 3.76 | 77 | 3.67 | 80 | 2.79 |
| Wound healing | 84 | 3.67 | 71 | 2.55 | 59 | 3.56 | 75 | 5.01 |
| Downregulation | ||||||||
| Cell cycle | 209 | 7.95 | 327 | 15.98 | 124 | 2.54 | 84 | 2.34 |
| Cell cycle checkpoint | 56 | 7.04 | 77 | 9.92 | 28 | 2.25 | 22 | 2.85 |
| Cell cycle phase | 154 | 9.09 | 248 | 17.46 | 79 | 2.22 | 54 | 2.11 |
| Cell cycle process | 168 | 8 | 275 | 16.69 | 95 | 2.37 | 64 | 2.12 |
| Cell division | 79 | 6.01 | 139 | 13.58 | 47 | 2.47 | 37 | 3.32 |
| Gene expression | 505 | 9.8 | 555 | 8.19 | 412 | 9.61 | 226 | 3.75 |
| M phase | 92 | 6.38 | 172 | 15.94 | 52 | 2.12 | 37 | 2.26 |
| M phase of mitotic cell cycle | 78 | 7.33 | 143 | 16.41 | 40 | 2.2 | 29 | 2.39 |
| Mitosis | 76 | 7.31 | 139 | 16.26 | 39 | 2.23 | 29 | 2.59 |
| Mitotic cell cycle | 151 | 10.08 | 233 | 17.53 | 76 | 2.72 | 50 | 2.17 |
| NcRNA metabolic process | 93 | 12.6 | 96 | 11.27 | 71 | 10.55 | 32 | 4.49 |
| NcRNA processing | 66 | 10.51 | 68 | 9.37 | 54 | 9.73 | 25 | 4.45 |
| Nuclear division | 76 | 7.31 | 139 | 16.26 | 39 | 2.23 | 29 | 2.59 |
| Nuclear export | 39 | 9.3 | 30 | 5.31 | 30 | 7.88 | 14 | 3.76 |
| Ribonucleoprotein complex assembly | 33 | 8.13 | 30 | 6.03 | 19 | 4.44 | 10 | 2.38 |
| Ribonucleoprotein complex biogenesis | 91 | 15.81 | 80 | 11.5 | 70 | 13.43 | 27 | 4.84 |
| Ribonucleoprotein complex subunit organization | 34 | 8.06 | 31 | 5.97 | 20 | 4.52 | 11 | 2.63 |
| Ribonucleotide metabolic process | 18 | 4.82 | 17 | 3.63 | 58 | 3.28 | 36 | 2.17 |
| Ribosome biogenesis | 69 | 15.54 | 60 | 11.38 | 61 | 15.71 | 20 | 4.84 |
| RNA processing | 172 | 13.95 | 180 | 12.43 | 126 | 10.82 | 52 | 3.11 |
| rRNA metabolic process | 47 | 11.72 | 44 | 9.39 | 41 | 11.7 | 17 | 4.98 |
| rRNA processing | 46 | 11.88 | 43 | 9.53 | 40 | 11.8 | 17 | 5.24 |
| Sterol biosynthetic process | 19 | 6.39 | 19 | 5.52 | 9 | 2.52 | 10 | 4.56 |
| Translation | 180 | 21.45 | 157 | 15.28 | 151 | 20.43 | 67 | 9.34 |
| Translational elongation | 75 | 19.97 | 55 | 12.1 | 77 | 23.76 | 32 | 11.05 |
| Translational initiation | 27 | 7.6 | 26 | 6.21 | 23 | 7.41 | 12 | 4.27 |
| Translational termination | 68 | 19.2 | 52 | 12.31 | 74 | 24.37 | 31 | 11.52 |
Specific ontologies were selected after analyses of nontransformed data. Data are reported for designated ontologies with ≥8 genes.