Table 11.
Effect of Serum Exposure on Phospholipid Fatty Acid Content in Human Primary and Immortalized Meibomian Gland Epithelial Cells
|
Phospholipid |
Serum-Free Media |
Serum-Containing Media |
||
|
Primary Cells |
Immortalized Cells |
Primary Cells |
Immortalized Cells |
|
| Phosphatidylcholine | ||||
| 38:2 | 2480, 2976 | 2798, 3794 | 1,795, 828.2 | 1,687, 1,168 |
| 38:4* | 567.2, 1206* | 699.4, 647.2* | 14,642, 9,083* | 5,157, 5,304* |
| 38:5 | 277.4, 629.3 | 338.3, 292.5 | 10,131, 6,107 | 4,206, 4,535 |
| 38:6 | 97.2, 156.1 | 66.0, 59.9 | 2,565, 1,674 | 1,169, 1,115 |
| Lysophosphatidylethanolamine | ||||
| 20:4* | 2155, 5363* | 2192, 3504* | 19,861, 12,622* | 11,603, 15,859* |
| 22:2 | 204.2, 386.6 | 364.0, 1091 | 75.6, 45.7 | 104.5, 77.5 |
| 22:4 | 312.6, 811.0 | 585.6, 1457 | 3,154, 2,254 | 2,220, 3,016 |
| 22:6 | 456.0, 1080 | 479.1, 916.8 | 21,845, 16,015 | 12,697, 14,728 |
| Lysophosphatidylserine | ||||
| 22:5 | 193.3, 521.9 | 240.6, 481.3 | 2,080, 1,383 | 1,449, 1,585 |
| 22:6 | 75.8, 222.9 | 103.6, 198.4 | 3,872, 2,435 | 2,111, 2,756 |
| Phosphatidylserine | ||||
| 40:6 | 56.7, 235.0 | 107.5, 160.8 | 2,836, 2,301 | 1,866, 1,897 |
| 40:7 | 17.8, 51.3 | 66.7, 25.0 | 221.3, 213.1 | 164.3, 131.4 |
| 42:9 | 0.4, 1.8 | 0.5, 0.8 | 118.8, 104.9 | 81.9, 79.6 |
| Phosphatidylethanolamine | ||||
| 38:2 | 312.8, 569.5 | 440.2, 618.0 | 167.0, 142.5 | 203.1, 118.4 |
| 38:4* | 330.0, 975.4* | 365.8, 404.6* | 2,970, 2,792* | 1,810, 1,753* |
| 38:6 | 30.7, 73.2 | 26.1, 26.9 | 551.7, 571.7 | 329.5, 321.0 |
| 40:7 | 17.0, 49.9 | 35.3, 35.3 | 925.7, 898.9 | 685.5, 616.5 |
| Lysophosphatidylglycerol | ||||
| 20:2 | 212.4, 823.6 | 585.1, 973.0 | 3,608, 1,574 | 1,550, 3,348 |
| 20:4* | 39.2, 131.8* | 119.3, 155.1* | 642.5, 277.9* | 232.3, 569.1* |
| 22:5 | 21.8, 85.0 | 24.7, 39.6 | 530.4, 205.6 | 232.2, 490.5 |
| 22:6 | 19.3, 75.2 | 18.8, 37.2 | 2,261, 1,029 | 970.4, 2,117 |
| Phosphatidylglycerol | ||||
| 40:7 | 0.6, 4.8 | 1.7, 3.9 | 117.1, 53.6 | 51.7, 97.0 |
Primary and immortalized human meibomian gland epithelial cells (n = 2 samples/condition) were cultured serum-free or serum-containing media and then processed for the analysis of fatty acids in the phospholipids. The numbers in the cell columns equal the amount (pg) of selected phospholipid fatty acids in each sample/condition.
*
The fatty acids represent (20:4), or may contain (38:4), arachidonic acid.