Figure 6.

Some RNA time traces show no excursions to an intermediate force state. As with Figure 5, red is the data and green is the offset quantized time trace. This trace shows no obvious excursions to an intermediate state. These excursions could be obscured by the noise. When we cluster the force levels of the time trace into three states, we recover a state very similar in magnitude to the high force state. This implies that k-means++ is having difficulty finding the low intermediate force state it had recovered in Figure 5.