Fig. 4.

Micrographs (A and B) and Western blot (C) showing the localization of activated NF-κB in SHRsp fed CT or Grn⁺ diet in heart ventricle (A) and kidney medulla (B and C) of 19-week-old animals. Positive nuclear NF-κB p65 signal was observed in ventricular endothelium and in the tubules of the kidney of SHRsp-CT with few positive cells seen in the SHRsp-Grn⁺ group. (C) Left shows a representative Western blot of nuclearly localized NF-κB p65 is shown on the left, whereas Right quantification of NF-κB p65 relative to β-actin (y axis depicts relative units). Data taken from kidney tissues of three animals per group. *, P < 0.05, for SHRsp-Grn⁺ versus SHRsp-CT.