Table 3.
Primers and Probes Used for Quantitative Reverse Transcription Polymerase Chain Reaction
Name | Gene/location | Sequence |
---|---|---|
Primer a (WL102) | Dystrophin exon 26 (forward primer) | 5′ gattttgaatataaaactccagatgaa |
Primer b (WL103) | Dystrophin exon 27 (reverse primer) | 5′ ggagtttcactttcgcttcttt |
Primer c (WL104) | Dystrophin exon 48 (forward primer) | 5′ aaccaaccaaaccaagaagg |
Primer d (WL105) | Dystrophin exon 49 (reverse primer) | 5′ gctgccctttagacaaaatctc |
Rplp0 forward primer | Ribosomal protein large P0 | 5′ ctccaagcagatgcagcaga |
Rplp0 reverse primer | Ribosomal protein large P0 | 5′ atagccttgcgcatcatggt |
Probe WL145 | The junction of the dystrophin head and body vectors | 5′ agagatgaagagagctaaagaagaggccc |
Probe WL146 | The junction of the dystrophin body and tail vectors | 5′ tttgacgttcaggaaactgaaatagcag |
Probe WL147 | The junction of the dystrophin head and tail vectors | 5′ agagatgaaggaaactgaaatagcagtt |
Rplp0 Probe | Ribosomal protein large P0 | 5′ ccgtggtgctgatgggcaagaa |