Table 1. ELISA of TGFβ2 in conditioned media.
| Active TGFβ2, pg/106 cells
|
Total TGFβ2, pg/106 cells
|
||||
|---|---|---|---|---|---|
| Conditioned media | Constitutively active NF-κB | Without antibody | With antibody | Without antibody | With antibody |
| No cells | — | 10 | 0 | 29 | 3 |
| 293C6 | — | 0 | 0 | 68 | 0 |
| WI38 | — | 0 | 0 | 85 | 9 |
| LNCaP | — | 0 | 0 | 100 | 16 |
| HT1080 | + | 0 | 0 | 370 | 0 |
| T98G | + | 48 | 16 | 1,700 | 76 |
| Mel29 | + | 0 | 0 | 510 | 0 |
| PC3 | + | 450 | 100 | 10,000 | 530 |
| DU145 | + | 27 | 0 | 920 | 0 |
| C6P1Z12 | + | 200 | 70 | 8,500 | 320 |
Media collected after 24 h were assayed with and without treatment with polyclonal anti-TGFβ2. Total TGFβ2 was assayed after exposure to 1 M HCl, to release latency-associated peptide. Active TGFβ2 was assayed without exposure to HCl.