Fig. 4.

Myelin-mediated axonal inhibition of ES cell-derived motoneurons in vitro. ES cells were plated on PLL/laminin-coated dishes that were then coated with myelin (A) or on PLL/laminin-coated dishes (B) 3 days after exposure to HhAg1.3/RA. Extensive axonal growth is seen only on PLL and laminin, suggesting that myelin inhibits axonal growth of ES cell-derived motoneurons. (C–E) Partial restoration of axonal growth when ES cell-derived motoneurons were plated on myelin in the presence of dbcAMP or Y-27632.