PNA increased afferent GABAergic drive to GnRH neurons. (A) Recordings of GABAergic PSCs from a representative GnRH neuron from a control female (Top), a PNA female (Middle), and a PNA female treated in vivo with flutamide (Bottom). (B) Mean ± SEM PSC frequency in GnRH neurons from control (n = 6 cells), PNA (n = 17), and PNA plus flutamide (n = 8) adult females. *, P < 0.05 versus control (Con).