Skip to main content
. 2004 Apr 21;101(18):7158–7163. doi: 10.1073/pnas.0401764101

Fig. 1.

Fig. 1.

Targeting of VGLUT1. (A) Targeting vector and VGLUT1 locus before and after homologous recombination. The horizontal arrow indicates the start codon. Coding exons 1–5 were replaced by a synaptobrevin 2-enhanced yellow fluorescent protein neomycin resistance (syb2-EYFP-neo) cassette, the positions of AseI restriction sites and 5′ Southern probe are marked. (B) Genomic Southern blot with 5′ probe after AseI digest of stem-cell DNA. The replacement of 6.2 kb of the VGLUT1 locus with the 2.5-kb synaptobrevin 2-enhanced yellow fluorescent protein neomycin resistance cassette results in a shift of the 8.4-kb WT band to 15.6 kb. (C) Genomic PCR with 280 bp for WT and 357 bp for targeted locus.