Skip to main content
. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Apr 24;50:82–91. doi: 10.1016/j.ibmb.2014.04.005

Fig. 2. Separation of the pooled hydroxylapatite fractions by gel filtration chromatography (A) and detection of proSPH processing by the column fractions (B).

Fig. 2

As described in Section 2.3, proteins in the flow-through fractions (#11-#26) were precipitated with solid ammonium sulfate, dissolved in 3 ml buffer, and resolved on a Sephacryl S100-HR column. IEARase activities in 10 µl fractions, A280nm values, and proSPH processing by the fractions (2 µl each) were measured and shown as described in Fig. 1 legend.