Skip to main content
. 2004 Apr 20;101(18):7193–7198. doi: 10.1073/pnas.0306665101

Fig. 1.

Fig. 1.

Effects of β2a on recombinant L-type channels reconstituted in HEK 293 cells. (A) (Left) Voltage protocol (Upper) and exemplar current (Lower) from a cell transfected with α1C-GFP alone (no β). Here and throughout, exemplar currents were evoked by a 20-ms test pulse to +50 mV from a holding potential of -100 mV; tail currents were analyzed at -50 mV repolarization. (Right) Population tail-activation (G–V) curve for no-β channels constructed from tail-current density (Jtail). (B) Exemplar current and G–V curve for cotransfection of α1C-GFP and β2a-subunits (▪). No β (○) is plotted for comparison; note difference in scale. (C) Exemplar ON-gating currents (Upper) and population ON-gating charge (QON, Lower). *, P < 0.001; number of cells in parentheses. (D) Plot of tail-current density at the reversal potential (Jrev) vs. QON for channels with β2a (▪) or no β (○). Lines are determined from linear regression of β2a (black) or no β (gray). (Inset) Detail for no β near the origin.