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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Nature. 2013 Dec 22;506(7486):111–115. doi: 10.1038/nature12824

Extended Data Figure 6. Recognition of RNA-DNA and DNA by hAptx.

Extended Data Figure 6

a, Structural overlays of DNA bound (blue-grey) and RNA-DNA bound (brown) hAptx complex structures. Inset: Interactions at the 5′ terminus reveal similar modes of engagement of RNA-DNA and DNA bound substrates. The β2-β3 loop residues Y195 and K197 orient the 5′ terminus for catalysis. b, Effects of β2-β3 loop mutants on Aptx deadenylation activity. Ten-fold dilutions of Aptx mutant proteins were tested for DNA adenylation activity on 5′-AMPRNA-DNA or 5′-AMPSSB substrates. Mean ± s.d. (n=2 technical replicates) is displayed. Fold increase to of protein to reach 50% activity is relative to the wild type hAptx for each substrate.