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Evaluation of transient transfection with follicle-stimulating hormone (FSH) siRNA in LCDE cells. FSH silencing was investigated by real-time PCR to evaluate the message of the protein (A) and by western blot to assess the protein expression of FSH (B). Using increasing amounts of siRNA FSH (0.25–1 μg), there was a significant reduction in FSH mRNA and protein expression.
