Figure 4. Transcription start site mapping of B. japonicum norC by FLOE.

Panel A corresponds to FLOE electropherograms obtained by using RNA from the wild type (upper panel) and regR mutant (bottom panel) both cultured anoxically in BSN medium. In panel B, RNA was isolated from the wild type (upper panel) and fixK ₂ mutant (botton panel) both cultured anoxically in YEM medium containing 10 mM KNO₃. In panel C, RNA was isolated from the wild type cultured in BSN medium under 2% initial O₂. The red peaks are GeneScan-500 ROXTM internal size markers. Filled blue peaks in each panel correspond to primer extension products P1 and P2.