Figure 4. Immunohistochemistry of the jejunum of one-day-old SPF chickens infected with the Shigella strain ZD02 via ligated intestinal loop.

Bacterial invasion (brown staining) was noticed in the epithelial cells 6 h (A) and 8 h (B) post-inoculation. The intestinal villus detached and the bacteria (brown staining) were released from the lysed epithelial cells (C). Intestinal villus of uninfected chicken was shown as control (D). Scale bars = 20 µm.