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Uninternalized bacteria (brown staining) were seen near the epithelial cells at 1 h post-inoculation (A). Internalized bacteria (brown staining) were found at 2 h (B) and 3 h (C). The lysed epithelial cells released bacteria (brown staining) at 4 h (D). Uninfected epithelial cells were shown as control (E). Scale bars = 20 µm.
