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. 2014 Jun 20;9(6):e100196. doi: 10.1371/journal.pone.0100196

Figure 1. Concentration-dependent change in MTT reduction.

Figure 1

Cell toxicity/proliferation was analyzed in neuron cultures at 48 h after treatment using an MTT assay. The findings were reported as percent of control absorbance at 540 nm (A540) ± SEM. Significance was analyzed using a one-way ANOVA and Duncan's post hoc test, from n  = 3 separate experiments. All treatment groups, except morphine alone [Control + Mor] and p24 = 10 pg/ml of HIV+ sup [HIV(10)], showed significantly decreased absorbance at 540 nm (*p<0.05 vs. Control), likely reflecting neurotoxicity. HIV+ sup caused a concentration-dependent reduction in A540 ($ p<0.05). Morphine did not show a significant interaction with HIV+ sup at any p24 level. Control  =  Controlsup; HIV  =  HIV+ sup (concentration of p24 in pg/ml is specified in parentheses); Mor  =  morphine sulfate (500 nM).