Table 1. Treatment paradigm for neurite growth/regrowth assessment.
Treatment Groups | Treatment from 0 to 24 h | Treatment from 24 to 72 h |
72 h (C) | Control | Control |
72 h (C+M) | Control + Mor | Control + Mor |
72 h (C+M+N) | Control + Mor + Nal | Control + Mor + Nal |
72 h (H) | HIV | HIV |
72 h (H+M) | HIV + Mor | HIV + Mor |
72 h (H+M+N) | HIV + Mor + Nal | HIV + Mor + Nal |
24 h (H) then 48 h (C) | HIV | Control |
24 h (H+M) them 48 h (C+M) | HIV + Mor | Control + Mor |
24 h (H+M+N) then 48 h (C+M+N) | HIV + Mor + Nal | Control + Mor + Nal |
Control = Controlsup; HIV = HIV+ sup (p24 = 25 pg/ml); Mor = morphine sulfate (500 nM); Nal = naloxone (1.5 µM).